Alterations in the Equine Herpesvirus Type‐1 (EHV‐1) Strain RacH During Attenuation
Identifieur interne : 003F94 ( Main/Exploration ); précédent : 003F93; suivant : 003F95Alterations in the Equine Herpesvirus Type‐1 (EHV‐1) Strain RacH During Attenuation
Auteurs : P. H. Hübert [Allemagne] ; S. Birkenmaier [Allemagne] ; H. Rziha [Allemagne] ; N. Osterrieder [Allemagne]Source :
- Journal of Veterinary Medicine, Series B [ 0931-1793 ] ; 1996-01-12.
English descriptors
- Teeft :
- Amino, Amino acids, Attenuation, Bamhi, Breeden, Colle, Deletion, Derivative, Equine, Equine herpesvirus, Equine herpesvirus rach, Equine herpesvirus type, Eusl, Fragment, Gene, Genome, Hamster, Herpes, Herpes simplex virus, Herpesvirus, Hybridization, Identical deletion, Kidney cells, Nucleotide, Nucleotide sequence, Osterrieder, Plaque, Rach, Rach raclll, Racll1, Raclll, Raclll rach, Restriction sites, Sequence analysis, Serial passages, Simplex, Strain rach, Telford, Transcriptional organization, Virol, Virology.
Abstract
The equine herpesvirus type‐1 modified live‐vaccine strain RacH (256th passage on porcine embryonic kidney cells) was investigated by restriction‐enzyme analysis and compared to representative plaque isolates of the 12th passage (RacL11, RacL22) and 185th passage (RacM24, RacM36). The restriction patterns of all Rac plaque isolates differed compared with reference strain Ab4. The left UL terminus was shortened by 0.1 kbp and a missing BamHI site led to the fusion of the f and t fragments. In some Rac derivatives, losses of restriction sites without deletions were observed: 1. One BamHI site located in the ribosyl reductase gene was missing in RacH, RacM24, RacM36, and RacL22; and 2. An SaiI site mapping to the gp14 (gB) gene was absent in RacM24, RacM36 and RacH. An identical deletion of 0.85 kbp in size was found in both copies of the inverted repeat (IR) regions of RacH. The deletion was present only in the terminal IR of the medium‐passage derivative RacM36. By contrast, in the genomes of the apathogenic RacM24, as well as the pathogenic plaque isolates RacL11 and RacL22, no deletions in the IRs were detectable. Nucleotide‐sequence and Northern‐blot analyses revealed that the deletions led to the elimination of one or both copies of the gene 67 (IR6) open‐reading frame in RacM36 and RacH and affected the gene 68 (EUS1) in RacH.
Url:
DOI: 10.1111/j.1439-0450.1996.tb00282.x
Affiliations:
- Allemagne
- Bade-Wurtemberg, Bavière, District de Haute-Bavière, District de Tübingen
- Munich, Tübingen
- Université Louis-et-Maximilien de Munich
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Birkenmaier</name><affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig‐Maximilian University of Munich</wicri:regionArea><placeName><settlement type="city">Munich</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region></placeName><orgName type="university">Université Louis-et-Maximilien de Munich</orgName></affiliation></author><author><name sortKey="Rziha, H" sort="Rziha, H" uniqKey="Rziha H" first="H." last="Rziha">H. Rziha</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Virus Diseases of Animals, Tübingen</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region><settlement type="city">Tübingen</settlement></placeName></affiliation></author><author><name sortKey="Osterrieder, N" sort="Osterrieder, N" uniqKey="Osterrieder N" first="N." last="Osterrieder">N. Osterrieder</name><affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig‐Maximilian University of Munich</wicri:regionArea><placeName><settlement type="city">Munich</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region></placeName><orgName type="university">Université Louis-et-Maximilien de Munich</orgName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Correspondence address: Institut für Medizinische Mikrobiologie, Infektions‐ und Seuchenmedizin, Veterinärstraße 13, D‐80539 München</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Veterinary Medicine, Series B</title><title level="j" type="alt">JOURNAL OF VETERINARY MEDICINE SERIES B</title><idno type="ISSN">0931-1793</idno><idno type="eISSN">1439-0450</idno><imprint><biblScope unit="vol">43</biblScope><biblScope unit="issue">1‐10</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="14">14</biblScope><biblScope unit="page-count">14</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1996-01-12">1996-01-12</date></imprint><idno type="ISSN">0931-1793</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0931-1793</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acids</term><term>Attenuation</term><term>Bamhi</term><term>Breeden</term><term>Colle</term><term>Deletion</term><term>Derivative</term><term>Equine</term><term>Equine herpesvirus</term><term>Equine herpesvirus rach</term><term>Equine herpesvirus type</term><term>Eusl</term><term>Fragment</term><term>Gene</term><term>Genome</term><term>Hamster</term><term>Herpes</term><term>Herpes simplex virus</term><term>Herpesvirus</term><term>Hybridization</term><term>Identical deletion</term><term>Kidney cells</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Osterrieder</term><term>Plaque</term><term>Rach</term><term>Rach raclll</term><term>Racll1</term><term>Raclll</term><term>Raclll rach</term><term>Restriction sites</term><term>Sequence analysis</term><term>Serial passages</term><term>Simplex</term><term>Strain rach</term><term>Telford</term><term>Transcriptional organization</term><term>Virol</term><term>Virology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The equine herpesvirus type‐1 modified live‐vaccine strain RacH (256th passage on porcine embryonic kidney cells) was investigated by restriction‐enzyme analysis and compared to representative plaque isolates of the 12th passage (RacL11, RacL22) and 185th passage (RacM24, RacM36). The restriction patterns of all Rac plaque isolates differed compared with reference strain Ab4. The left UL terminus was shortened by 0.1 kbp and a missing BamHI site led to the fusion of the f and t fragments. In some Rac derivatives, losses of restriction sites without deletions were observed: 1. One BamHI site located in the ribosyl reductase gene was missing in RacH, RacM24, RacM36, and RacL22; and 2. An SaiI site mapping to the gp14 (gB) gene was absent in RacM24, RacM36 and RacH. An identical deletion of 0.85 kbp in size was found in both copies of the inverted repeat (IR) regions of RacH. The deletion was present only in the terminal IR of the medium‐passage derivative RacM36. By contrast, in the genomes of the apathogenic RacM24, as well as the pathogenic plaque isolates RacL11 and RacL22, no deletions in the IRs were detectable. Nucleotide‐sequence and Northern‐blot analyses revealed that the deletions led to the elimination of one or both copies of the gene 67 (IR6) open‐reading frame in RacM36 and RacH and affected the gene 68 (EUS1) in RacH.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>Bavière</li><li>District de Haute-Bavière</li><li>District de Tübingen</li></region><settlement><li>Munich</li><li>Tübingen</li></settlement><orgName><li>Université Louis-et-Maximilien de Munich</li></orgName></list><tree><country name="Allemagne"><region name="Bavière"><name sortKey="Hubert, P H" sort="Hubert, P H" uniqKey="Hubert P" first="P. H." last="Hübert">P. H. Hübert</name></region><name sortKey="Birkenmaier, S" sort="Birkenmaier, S" uniqKey="Birkenmaier S" first="S." last="Birkenmaier">S. Birkenmaier</name><name sortKey="Osterrieder, N" sort="Osterrieder, N" uniqKey="Osterrieder N" first="N." last="Osterrieder">N. Osterrieder</name><name sortKey="Osterrieder, N" sort="Osterrieder, N" uniqKey="Osterrieder N" first="N." last="Osterrieder">N. Osterrieder</name><name sortKey="Rziha, H" sort="Rziha, H" uniqKey="Rziha H" first="H." last="Rziha">H. Rziha</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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